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Entire Genome Sequencing from Single Molecule DNA
http://microarray.wordpress.com/ 2008/ 04/ 11/ entire-genome-sequencing-from-single-...
Cambridge based Helicos Bioscience announced the publication of a report in Science Magazine demonstrating the first single molecule sequencing of an organism (M13 virus genome) examining more than 280,000 strands of captured DNA. Helicos’ uses a proprietary form of sequencing-by-synthesis called True Single Molecule Sequencing(tSMS)™. Unlike other methods, the technique builds up the sequence of each strand of DNA one base at a time. Filed under: DNA, microarray blog, sequencing
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CQNCer , players in dna sequencing
http://computationalbiologynews.blogspot.com/2008/06/cqncer-...So finally we see Helicos in action (via http://microarray.wordpress.com/2008/04/11/entire-genome-sequencing-from-single-molecule-dna/ ) , http://www.sciencemag.org/cgi/content/full/320/5872/106 . They have sequenced the M13 phage virus (about .3 mill bases) ... sometime back I was surveying the trends in sequencing and captured them at http://www.slideshare.net/sharma_animesh/cqncer . These strategies are generally being used for reqsequencing or sequencing smaller genomes. Though I see that even smaller genomes like Stenotrophomonas maltophilia ( Steno, http://genomebiology.com/2008/9/4/R74 ) done the old way... more at http://www.biologynews.net/archives/2008/05/07/superbug_genome_sequenced.html . Same goes for recently sequenced Platypus gnome ( http://www.nature.com/nature/journal/v453/n7192/abs/nature06936.html , http://www.eurekalert.org/pub_releases/2008-05/embl-pgs050608.php ) and the Papaya genome draft ( http://www.nature.com/nature/journal/v452/n7190/abs/nature06856.html , http://www.biologynews.net/archives/2008/05/14/scientists_decipher_fruit_tree_genome_for_the_first_time.html ) but given these new cheaper sequencing technologies and better algorithms, we are bound to see cooler changes in Tree-of-Life ( http://www.boston.com/news/science/articles/2008/04/28/tree_of_life_continues_to_evolve/ ) ! clipped from www.ncbi.nlm.nih.gov The Genome database provides views for a variety of genomes, complete chromosomes, sequence maps with contigs, and integrated genetic and physical maps. The database is organized in six major organism groups: Archaea, Bacteria, Eukaryotae, Viruses, Viroids, and Plasmids and includes complete chromosomes, organelles and plasmids as well as draft genome assemblies.
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